Use of a small palindrome genetic marker to investigate mechanisms of double-strand-break repair in mammalian cells.

@article{Li2000UseOA,
  title={Use of a small palindrome genetic marker to investigate mechanisms of double-strand-break repair in mammalian cells.},
  author={Jianjun Li and Mark David Baker},
  journal={Genetics},
  year={2000},
  volume={154 3},
  pages={1281-9}
}
We examined mechanisms of mammalian homologous recombination using a gene targeting assay in which the vector-borne region of homology to the chromosome bore small palindrome insertions that frequently escape mismatch repair when encompassed within heteroduplex DNA (hDNA). Our assay permitted the product(s) of each independent recombination event to be recovered for molecular analysis. The results revealed the following: (i) vector-borne double-strand break (DSB) processing usually did not… CONTINUE READING

References

Publications referenced by this paper.
Showing 1-10 of 12 references

Palin - drome resolution and recombination in the mammalian germ in DNA form stable hairpins in vivo in S . cerevisiae

  • J. Zahn, S. Baumes, G. Brown
  • 1997

A test of the double - strand Saccharomyces cerevisiae does not represent a site for a symmetricallybreak repair model for meiotic recombination in Saccharomyces processed double - strand break

  • M. A. Resnick, G. I. Goldberg
  • Genetics
  • 1996

Measurements of excision repair nisms of overlap formation in nonhomologous end joining

  • L. A. Gilbertson, F. W. Stahl
  • Mol . Cell . Biol . Mol . Cell . Biol .
  • 1992

The role and fate ing : A Laboratory Manual , Ed . 2 . , Cold Spring Harbor Laboratoryof DNA ends for homologous recombination in embryonic stem Press , Cold Spring Harbor , NY . cells

  • T. Maniatis
  • Eur . J . Mol . Genet .
  • 1992

Loss of consti - break in Saccharomyces cerevisiae

  • W. Cavenee, M. Nordenskjöld
  • Genetics
  • 1991

Immunoglobulin M mutants . tract repair of palindromic loop mismatches in mammalian cells

  • D. G. Taghian, H. Hough, J. A. Nickoloff, G. Köhler, M. J. Shulman
  • Eur . J . Immunol .
  • 1980

Similar Papers

Loading similar papers…