Use of a simple method to isolate intact RNA from partially hydratedSelaginella lepidophylla plants

@article{ValenzuelaAvendao2007UseOA,
  title={Use of a simple method to isolate intact RNA from partially hydratedSelaginella lepidophylla plants},
  author={Juan P. Valenzuela-Avenda{\~n}o and Iv{\'a}n A. Estrada Mota and Gabriel Lizama Uc and Ram{\'o}n Souza Perera and Elisa Miriam Valenzuela-Soto and Jos{\'e} J. Z{\'u}{\~n}iga Aguilar},
  journal={Plant Molecular Biology Reporter},
  year={2007},
  volume={23},
  pages={199-200}
}
Isolation of high-quality RNA is a necessary step in gene expression analysis. Although many methods can be used to isolate RNA from plants where contamination of preparations with complex carbohydrates or phenolic compounds is a problem, the application of these methods toSelaginella lepidophylla tissues has failed to obtain good-quality RNA. Here we introduce 2 modifications to the method developed by Chomczynski and Sacchi (1987), generating a simple and rapid method that allows the… CONTINUE READING