Use of a selectable marker regulated by alpha interferon to obtain mutations in the signaling pathway.

@article{Pellegrini1989UseOA,
  title={Use of a selectable marker regulated by alpha interferon to obtain mutations in the signaling pathway.},
  author={Silvia Pellegrini and Joseph K John and Michael Shearer and Ian M. Kerr and George R Stark},
  journal={Molecular and cellular biology},
  year={1989},
  volume={9 11},
  pages={4605-12}
}
We have selected mutations in genes encoding components of the signaling pathway for alpha interferon (IFN-alpha) by using a specially constructed cell line. The upstream region of the IFN-regulated human gene 6-16 was fused to the Escherichia coli guanine phosphoribosyltransferase (gpt) gene and transfected into hypoxanthine-guanine phosphoribosyltransferase-negative human cells. These cells express gpt only in the presence of IFN-alpha. They grow in medium containing hypoxanthine, aminopterin… CONTINUE READING

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