Use of a recombinant retrovirus to study post-implantation cell lineage in mouse embryos.

@article{Sanes1986UseOA,
  title={Use of a recombinant retrovirus to study post-implantation cell lineage in mouse embryos.},
  author={Joshua R Sanes and John L. Rubenstein and Jean François Nicolas},
  journal={The EMBO journal},
  year={1986},
  volume={5 12},
  pages={3133-42}
}
We show that a gene introduced into cells of mouse embryos by a retrovirus can serve as a heritable marker for the study of cell lineage in vivo. We constructed a defective recombinant retrovirus in which the Escherichia coli beta-galactosidase (lacZ) gene is inserted in the genome of a Muloney murine leukemia virus (M-MuLV). Expression of lacZ was detected with a histochemical stain that can be applied to cultured cells and embryonic tissue. Infection of cultured cells showed that lacZ has no… CONTINUE READING
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