Use of a recombinant 170-kilodalton surface antigen of Entamoeba histolytica for serodiagnosis of amebiasis and identification of immunodominant domains of the native molecule.

@article{Zhang1992UseOA,
  title={Use of a recombinant 170-kilodalton surface antigen of Entamoeba histolytica for serodiagnosis of amebiasis and identification of immunodominant domains of the native molecule.},
  author={Yunhong Zhang and Erguang Li and Thomas Herbert Elliot Jackson and Tao Zhang and Vinodh. Gathiram and Samuel Stanley},
  journal={Journal of clinical microbiology},
  year={1992},
  volume={30 11},
  pages={
          2788-92
        }
}
We expressed the gene that encodes one of the major surface antigens of Entamoeba histolytica, the 170-kDa protein (1,270 amino acids), as a glutathione S-transferase fusion protein containing amino acids 1 to 1202 (lacking the putative transmembrane and cytoplasmic regions) and as separate fusion proteins containing each of three major domains of the 170-kDa molecule. Lysates from bacteria induced to express one of these proteins were used as the target antigens in a Western blot (immunoblot… CONTINUE READING

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