Use of a real-time polymerase chain reaction-based fluorogenic 5' nuclease assay to evaluate insect vectors of Corynebacterium pseudotuberculosis infections in horses.

@article{Spier2004UseOA,
  title={Use of a real-time polymerase chain reaction-based fluorogenic 5' nuclease assay to evaluate insect vectors of Corynebacterium pseudotuberculosis infections in horses.},
  author={Sharon J. Spier and Christian M. Leutenegger and Scott P. Carroll and Jenella E. Loye and Jeannine Berger Pusterla and Tim E. Carpenter and Judy E Mihalyi and John E. Madigan},
  journal={American journal of veterinary research},
  year={2004},
  volume={65 6},
  pages={829-34}
}
OBJECTIVE To develop and use a sensitive molecular assay for detecting the phospholipase D (PLD) exotoxin gene of Corynebacterium pseudotuberculosis in an attempt to identify insect vectors that may be important in transmission of clinical disease in horses. SAMPLE POPULATION 2,621 flies of various species. PROCEDURE A real-time polymerase chain reaction (PCR)-based fluorogenic 5' nuclease (TaqMan) system (ie, TaqMan PCR assay) was developed for the detection of the PLD gene in insects… CONTINUE READING
16 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 16 extracted citations

Similar Papers

Loading similar papers…