Use of a randomized hybrid ribozyme library for identification of genes involved in muscle differentiation.

@article{Wadhwa2004UseOA,
  title={Use of a randomized hybrid ribozyme library for identification of genes involved in muscle differentiation.},
  author={Renu Wadhwa and Tomoko Yaguchi and Kamaljit Kaur and Eigo Suyama and Hiroyuki Kawasaki and K. Taira and Sunil C. Kaul},
  journal={The Journal of biological chemistry},
  year={2004},
  volume={279 49},
  pages={51622-9}
}
We have employed the hybrid hammerhead ribozyme-based gene discovery system for identification of genes functionally involved in muscle differentiation using in vitro myoblast differentiation assay. The major muscle regulatory genes (MyoD1, Mylk, myosin, myogenin, and Myf5) were identified endorsing the validity of this method. Other gene targets included tumor suppressors and cell cycle regulators (p19ARF and p21WAF1), FGFR-4, fibronectin, Prkg2, Pdk4, fem, and six novel proteins. Functional… CONTINUE READING

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