Use of a quantitative real-time reverse transcription-polymerase chain reaction method to study the induction of CYP1A, CYP2B and CYP4A forms in precision-cut rat liver slices.

@article{Pan2002UseOA,
  title={Use of a quantitative real-time reverse transcription-polymerase chain reaction method to study the induction of CYP1A, CYP2B and CYP4A forms in precision-cut rat liver slices.},
  author={Jinmei Pan and Qian Xiang and Anthony B. Renwick and Roger J. Price and Simon E Ball and John Kao and Joann Scatina and Brian G Lake},
  journal={Xenobiotica; the fate of foreign compounds in biological systems},
  year={2002},
  volume={32 9},
  pages={739-47}
}
1. The aim was to employ real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) technology (TaqMan to examine the induction of some selected cytochrome P450 (CYP) forms in precision-cut rat liver slices. 2. Taqman primers and probe sets were developed for rat CYP1A1, CYP1A2, CYP2B1 and CYP4A1 forms. 3. Rat liver slices were cultured in control medium or medium containing either 10 micro g ml(-1) Aroclor 1254 (ARO), 500 micro M sodium phenobarbitone (NaPB) or 50 micro M… CONTINUE READING

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