Use of a phage vector for rapid synthesis and cloning of single-stranded cDNA.

Abstract

We have developed a technique for synthesis of single stranded complementary DNA (ss cDNA) using specifically designed phage ssDNA as vector primer. This vector (pPBS27) was constructed by introducing a poly(dT) tail adjacent to the XbaI site of pTZ18R, which can exist either as a plasmid in Escherichia coli or as a ssDNA phage. The pPBS27 phage vector is… (More)

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Cite this paper

@article{Bellemare1987UseOA, title={Use of a phage vector for rapid synthesis and cloning of single-stranded cDNA.}, author={Guy Bellemare and Claude L. Potvin and Claire Simard and Lucie Larouche}, journal={Gene}, year={1987}, volume={52 1}, pages={11-9} }