Use of a novel washing method combining multiple density gradients and trypsin for removing human immunodeficiency virus-1 and hepatitis C virus from semen.

@article{Loskutoff2005UseOA,
  title={Use of a novel washing method combining multiple density gradients and trypsin for removing human immunodeficiency virus-1 and hepatitis C virus from semen.},
  author={Naida M Loskutoff and Carin Huyser and Raksha Singh and David L. Walker and Alan Russell Thornhill and Lynn Morris and Lynne Webber},
  journal={Fertility and sterility},
  year={2005},
  volume={84 4},
  pages={
          1001-10
        }
}
OBJECTIVE To determine the effectiveness of a novel treatment designed to remove human immunodeficiency virus (HIV) -1 and hepatitis C virus (HCV) from spiked semen and to evaluate sperm motility and viability after treatment. DESIGN A prospective clinical laboratory-based study. SETTING The human studies were conducted in academic and national research environments. The bovine study was conducted in an accredited research facility. PATIENT(S) Healthy volunteers provided the semen samples… CONTINUE READING

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