Use of a novel Förster resonance energy transfer method to identify locations of site-bound metal ions in the U2–U6 snRNA complex

@inproceedings{Yuan2007UseOA,
  title={Use of a novel F{\"o}rster resonance energy transfer method to identify locations of site-bound metal ions in the U2–U6 snRNA complex},
  author={Faqing Yuan and Laura Griffin and LauraJane Phelps and Volker Buschmann and Kenneth D. Weston and Nancy L. Greenbaum},
  booktitle={Nucleic acids research},
  year={2007}
}
U2 and U6 snRNAs pair to form a phylogenetically conserved complex at the catalytic core of the spliceosome. Interactions with divalent metal ions, particularly Mg(II), at specific sites are essential for its folding and catalytic activity. We used a novel Förster resonance energy transfer (FRET) method between site-bound luminescent lanthanide ions and a covalently attached fluorescent dye, combined with supporting stoichiometric and mutational studies, to determine locations of site-bound Tb… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-8 of 8 extracted citations

Similar Papers

Loading similar papers…