Use of a new tetrazolium-based assay to study the production of superoxide radicals by tobacco cell cultures challenged with avirulent zoospores of phytophthora parasitica var nicotianae

@article{Able1998UseOA,
  title={Use of a new tetrazolium-based assay to study the production of superoxide radicals by tobacco cell cultures challenged with avirulent zoospores of phytophthora parasitica var nicotianae},
  author={Able and Guest and Sutherland},
  journal={Plant physiology},
  year={1998},
  volume={117 2},
  pages={491-9}
}
The relationship between the production of reactive oxygen species and the hypersensitive response (HR) of tobacco (Nicotiana tabacum L. ) toward an incompatible race of the Oomycete Phytophthora parasitica var nicotianae has been investigated. A new assay for superoxide radical (O2-) production based on reduction of the tetrazolium dye sodium,3'-(1-[phenylamino-carbonyl]-3, 4-tetrazolium)-bis(4-methoxy-6-nitro) benzene-sulfonic acid hydrate (XTT) has enabled the quantitative estimation of… CONTINUE READING