Use of a new fluorescent probe, seminaphthofluorescein-calcein, for determination of intracellular pH by simultaneous dual-emission imaging laser scanning confocal microscopy.

@article{Zhou1995UseOA,
  title={Use of a new fluorescent probe, seminaphthofluorescein-calcein, for determination of intracellular pH by simultaneous dual-emission imaging laser scanning confocal microscopy.},
  author={Yuxiang Zhou and Elliott M. Marcus and Richard P. Haugland and M. Opas},
  journal={Journal of cellular physiology},
  year={1995},
  volume={164 1},
  pages={9-16}
}
A new pH indicator, seminaphthofluorescein (SNAFL)-calcein acetoxymethyl ester, was used for intracellular pH (pHi) measurement in living MDCK cells with a laser scanning confocal microscope (LSCM) equipped with an Argon/Krypton laser and dual-excitation and dual-emission (FITC/Texas Red) filter set. SNAFL-calcein excitation maxima are approximately 492/540 nm (acid/base) and emission maxima are approximately 535/625 nm (acid/base) with a pKa value at approximately 7.0. The absorption/emission… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-8 of 8 extracted citations

Vestibular hair bundles control pH with (Na+, K+)/H+ exchangers NHE6 and NHE9.

The Journal of neuroscience : the official journal of the Society for Neuroscience • 2006
View 4 Excerpts
Highly Influenced

Novel Methods in Molecular and Cellular Biochemistry of Muscle

Developments in Molecular and Cellular Biochemistry • 1997

Similar Papers

Loading similar papers…