Use of a mixture of glucose and methanol as substrates for the production of recombinant trypsinogen in continuous cultures with Pichia pastoris Mut+.

@article{Paulov2012UseOA,
  title={Use of a mixture of glucose and methanol as substrates for the production of recombinant trypsinogen in continuous cultures with Pichia pastoris Mut+.},
  author={Leona Paulov{\'a} and Petr Hyka and Barbora Bransk{\'a} and Karel Melzoch and Karin Kovar},
  journal={Journal of biotechnology},
  year={2012},
  volume={157 1},
  pages={180-8}
}
Pure methanol, which is required as an inducer of the AOX1 promoter and a carbon/energy source in processes for recombinant protein production by Pichia pastoris, is impracticable and therefore generally undesirable. As an alternative, a procedure using double carbon substrate was examined (11.7g(carbon)l(-1), 60%/40% carbon from glucose/methanol). The effects on methanol metabolism, extracellular formation of porcine trypsinogen, biomass growth and cell viability were analyzed. In contrast to… CONTINUE READING

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