Use of a mammalian internal ribosomal entry site element for expression of a foreign protein by a transfectant influenza virus.

@article{GarcaSastre1994UseOA,
  title={Use of a mammalian internal ribosomal entry site element for expression of a foreign protein by a transfectant influenza virus.},
  author={Adolfo Garc{\'i}a-Sastre and Thomas Muster and Wendy S Barclay and N S Percy and P. Palese},
  journal={Journal of virology},
  year={1994},
  volume={68 10},
  pages={6254-61}
}
The ribonucleoprotein transfection system for influenza virus allowed us to construct two influenza A viruses, GP2/BIP-NA and HGP2/BIP-NA, which contained bicistronic neuraminidase (NA) genes. The mRNAs derived from the bicistronic NA genes have two different open reading frames (ORFs). The first ORF encodes a foreign polypeptide (GP2 or HGP2) containing amino acid sequences derived from the gp41 protein of human immunodeficiency virus type 1. The second ORF encodes the NA protein; its… CONTINUE READING