Use of a locked-nucleic-acid oligomer in the clamped-probe assay for detection of a minority Pfcrt K76T mutant population of Plasmodium falciparum.

@article{Senescau2005UseOA,
  title={Use of a locked-nucleic-acid oligomer in the clamped-probe assay for detection of a minority Pfcrt K76T mutant population of Plasmodium falciparum.},
  author={Alice Senescau and Antoine Berry and Françoise Benoit-Vical and Olfert Landt and Richard Fabre and J{\"o}el Leli{\`e}vre and Sophie Cassaing and J. -F. Magnaval},
  journal={Journal of clinical microbiology},
  year={2005},
  volume={43 7},
  pages={3304-8}
}
Given the emergence of drug resistance and the high rate of polyclonal microorganism infections, the availability of a fast and sensitive test to detect minority mutant populations would be an improvement in the diagnosis of infectious diseases. A clamped-probe real-time PCR assay to diagnose the Plasmodium falciparum K76T mutation in clone populations was developed, using a wild-type-specific locked-nucleic-acid-containing oligomer to suppress wild-type PCR amplification and to enhance melting… CONTINUE READING

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