Use of a hybrid vaccinia virus-T7 RNA polymerase system for expression of target genes.

  title={Use of a hybrid vaccinia virus-T7 RNA polymerase system for expression of target genes.},
  author={Thomas R. Fuerst and Patricia L. Earl and Bernard Moss},
  journal={Molecular and cellular biology},
  volume={7 7},
A novel expression system based on coinfection of cells with two recombinant vaccinia viruses has been developed. One recombinant vaccinia virus contained the bacteriophage T7 RNA polymerase gene under control of a vaccinia virus promoter. The second recombinant vaccinia virus contained a target gene of choice flanked by bacteriophage T7 promoter and termination sequences. Maximum expression of the target gene occurred when cells were infected with 10 PFU of each recombinant virus. Although T7… CONTINUE READING
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