Use of a human minichromosome as a cloning and expression vector for mammalian cells.

@article{Guiducci1999UseOA,
  title={Use of a human minichromosome as a cloning and expression vector for mammalian cells.},
  author={Cristiana Guiducci and Fiorentina Ascenzioni and Cristina Auriche and Enza Piccolella and Anna Maria Guerrini and Pierluigi Donini},
  journal={Human molecular genetics},
  year={1999},
  volume={8 8},
  pages={
          1417-24
        }
}
A natural human minichromosome (MC1) derived from human chromosome 1 was shown to be linear and to have a size of 5.5 Mb. Human IL-2 cDNA and the neo gene were co-transfected into a MC1-containing human-CHO hybrid cell line. Integration of the foreign genes was directed to the pericentromeric region of MC1 by co-transfection of chromosome 1-specific satellite 2 DNA. A number of G418-resistant transfectants were obtained and expression of IL-2 was determined. FISH analysis demonstrated co… Expand
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References

SHOWING 1-10 OF 49 REFERENCES
Gene targeting to the centromeric DNA of a human minichromosome.
TLDR
The supernumerary minichromosome, previously identified as a derivative of chromosome 9, has been introduced into Chinese hamster ovary cells by means of cell fusion and may represent a model for the construction of a large-capacity vector for somatic gene therapy. Expand
Extrachromosomal maintenance and amplification of yeast artificial chromosome DNA in mouse cells.
TLDR
The fusion cell lines contain the two characteristic forms of amplified DNA observed in cancer cell lines, DMs and HSRs, indicating that these can be formed from fragments of DNA introduced by cell fusion. Expand
Molecular characterization of human minichromosomes with centromere from chromosome 1 in human-hamster hybrid cells
TLDR
Examining the amounts of four different human satellite DNA sequences in a series of human-hamster hybrid cells suggests that there may be redundancy in the system that allows for a variation in the size of the kinetochore and the number of attachment sites for microtubules. Expand
Stable episomal maintenance of yeast artificial chromosomes in human cells
TLDR
Fluorescence in situ hybridization analysis demonstrated a close association of OriPYACs, some of which were visible as pairs, with host cell chromosomes, suggesting that the episomes replicate once per cell cycle and that stability is achieved by attachment to host chromosomes, as suggested for the viral genome. Expand
Generation of a human X‐derived minichromosome using telomere‐associated chromosome fragmentation.
TLDR
In situ hybridization and molecular analysis reveal that the minichromosome has a linear structure, with two introduced telomere constructs flanking a 2.5 Mb alpha‐satellite array. Expand
Radiation fusion hybrids for human chromosomes 3 and X generated at various irradiation doses
TLDR
Upon further characterization, the 86 hybrids analyzed here will provide a mapping panel for the entire chromosomes 3 and X with an estimated resolution in the range of 1–2 Mb on average, a size range amenable to PFGE and YAC contig mapping. Expand
Engineering mammalian chromosomes.
TLDR
Report on two reports showing that stable, low copy number MACs containing a functional centromere can be produced following transfection of naked DNA into the human HT1080 cell line, demonstrating for the first time that alpha-satellite DNA can seed de novo centromeres in human cells. Expand
Human artificial episomal chromosomes for cloning large DNA fragments in human cells
TLDR
The autologous HAEC system, with human DNA cloned directly in human cells, provides an important tool for functional study of large mammalian DNA regions and gene therapy. Expand
Sandwiching of a gene within 12 kb of a functional telomere and alpha satellite does not result in silencing.
TLDR
This study indicates that structural elements flanking a mammalian selectable marker do not result in silencing and the truncated chromosome has a fully functional mitotic centromere. Expand
Formation of de novo centromeres and construction of first-generation human artificial microchromosomes
TLDR
This first-generation system for the construction of human artificial chromosomes should be suitable for dissecting the sequence requirements of human centromeres, as well as developing constructs useful for therapeutic applications. Expand
...
1
2
3
4
5
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