Use of a human X mouse hybrid cell line to detect aneuploidy induced by environmental chemicals.

@article{Athwal1985UseOA,
  title={Use of a human X mouse hybrid cell line to detect aneuploidy induced by environmental chemicals.},
  author={Raghbir S. Athwal and Shahbeg S. Sandhu},
  journal={Mutation research},
  year={1985},
  volume={149 1},
  pages={
          73-81
        }
}
A short-term assay utilizing a human/mouse monochromosomal hybrid cell line R3-5, to detect chemically induced aneuploidy in mammalian cells is described. A single human chromosome transferred into mouse cells was used as a cytogenetic marker to quantitate abnormal chromosome segregation following chemical treatment. The human chromosome present in the mouse cells can be readily identified by differential staining procedures. The frequency of cells containing 0 or 2 human chromosomes in the… CONTINUE READING
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