Use of a heterodimeric coiled-coil system for biosensor application and affinity purification.

@article{Chao1998UseOA,
  title={Use of a heterodimeric coiled-coil system for biosensor application and affinity purification.},
  author={Heman Chao and Debra L. Bautista and Jennifer R. Litowski and Randall T. Irvin and Robert S. Hodges},
  journal={Journal of chromatography. B, Biomedical sciences and applications},
  year={1998},
  volume={715 1},
  pages={307-29}
}
The two-stranded alpha-helical coiled-coil is now recognized as one of nature's favorite ways of creating a dimerization motif. Based on the knowledge of protein folding studies and de novo design model systems, a novel heterodimeric coiled-coil protein was synthesized. The heterodimeric E/K coiled-coil was constructed with two distinct peptides (E and K… CONTINUE READING