Use of a genetically encoded hydrogen peroxide sensor for whole cell screening of enzyme activity.

Abstract

We report the use of HyPer, a genetically encoded, fluorescent sensor that reacts with hydrogen peroxide (H2O2), in a novel screen to engineer enzymes for enhanced production of H2O2. We co-expressed HyPer with cytochrome P450 BM3 variants and, using HyPer's ratiometric signal, found variants that produce greater amounts of H2O2 than the wild-type enzyme… (More)
DOI: 10.1093/protein/gzv003

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