Use of a dual firefly and Renilla luciferase reporter gene assay to simultaneously determine drug selectivity at human corticotrophin releasing hormone 1 and 2 receptors.

@article{Parsons2000UseOA,
  title={Use of a dual firefly and Renilla luciferase reporter gene assay to simultaneously determine drug selectivity at human corticotrophin releasing hormone 1 and 2 receptors.},
  author={Sarah J Parsons and Sharron A Rhodes and Helen E Connor and Stephen A. Rees and Jez Brown and Heather Giles},
  journal={Analytical biochemistry},
  year={2000},
  volume={281 2},
  pages={187-92}
}
The aim of this study was to investigate and validate the use of a dual glow-signal luciferase reporter gene assay to simultaneously evaluate drug activity at two different seven-transmembrane receptor subtypes. Stable cell lines (CHO) transfected with either human corticotrophin releasing hormone 1 (hCRH1) receptors and a firefly luciferase reporter gene… CONTINUE READING