Use of a cellulase-encoding gene probe to reveal restriction fragment length polymorphisms among ruminal strains ofBacteroides succinogenes

A cloned DNA fragment specifying an endoglucanase fromBacteroides succinogenes strain BL2 was shown to hybridize under nonstringent conditions to different BamH1 fragments of chromosomal DNA from each of five rumen strains ofB. succinogenes. Direct binding of BL2 total chromosomal DNA to DNA from the other four strains was between 16% and 42% of homologous… CONTINUE READING