Use of a blocking primer allows selective amplification of bacterial DNA from microalgae cultures.

Abstract

The production of algae is a crucial component of many aquaculture systems and the role of bacteria in this process is an important although complex one. We report the development of a new blocking primer that allowed PCR amplification of bacterial DNA in the presence of algal chloroplast DNA.

DOI: 10.1016/j.mimet.2012.05.007

Cite this paper

@article{Powell2012UseOA, title={Use of a blocking primer allows selective amplification of bacterial DNA from microalgae cultures.}, author={Shane M. Powell and Christopher C Chapman and Michel Bermudes and Mark L. Tamplin}, journal={Journal of microbiological methods}, year={2012}, volume={90 3}, pages={211-3} }