Use of a biotinyl-estradiol derivative to demonstrate estradiol-membrane binding sites on adherent human breast cancer MCF-7 cells.

@article{Germain1993UseOA,
  title={Use of a biotinyl-estradiol derivative to demonstrate estradiol-membrane binding sites on adherent human breast cancer MCF-7 cells.},
  author={Paul Germain and Philippe M{\'e}t{\'e}zeau and Louis X Tiefenauer and H{\'e}l{\`e}ne Kiefer and Marie H{\'e}l{\`e}ne Ratinaud and G{\'e}rard Habrioux},
  journal={Anticancer research},
  year={1993},
  volume={13 6A},
  pages={2347-53}
}
A biotinyl-derivative of 17 beta-estradiol has been used to demonstrate a site of recognition and binding of estradiol located on the plasma membrane of human breast cancer MCF-7 cells by using the biotin/avidin-FITC system. The specificity of this binding has been shown by a displacement of the fluorescent label by 17 beta-estradiol. No displacement was observed when testosterone was added. Quantification of this phenomenon has been shown by laser scanning cytometry while preserving the cells… CONTINUE READING

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