Use of a PCR method on fecal samples for diagnosis of sheep paratuberculosis.

@article{Garrido2000UseOA,
  title={Use of a PCR method on fecal samples for diagnosis of sheep paratuberculosis.},
  author={J. M. Beltr{\'a}n Garrido and Natalia Cortabarr{\'i}a and Jos{\'e} A. Oguiza and Gorka Ad{\'u}riz and Ram{\'o}n A Juste},
  journal={Veterinary microbiology},
  year={2000},
  volume={77 3-4},
  pages={
          379-86
        }
}
The high sensitivity of PCR compared to the difficulties of fecal culture in sheep prompted the development of PCR protocols for detection of Mycobacterium avium subsp. paratuberculosis DNA in sheep feces. Although the PCR itself is well developed, and does not pose large technical problems, concentrating the bacteria from samples that may contain low numbers of bacilli using practical methods is still the main difficulty for the use of this technique. In this study, we describe an extraction… CONTINUE READING
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