Use of a Closed Culture System to Improve the Safety of Lentiviral Vector Production.

@article{Wu2015UseOA,
  title={Use of a Closed Culture System to Improve the Safety of Lentiviral Vector Production.},
  author={Tao Wu and Ga{\"e}tan Bour and Sarah C. Durand and V{\'e}ronique Lindner and Francine Goss{\'e} and Laetitia Zona and Jean-Marie Certoux and Michele Diana and Thomas F Baumert and Jacques Marescaux and Didier Mutter and Patrick Pessaux and Eric Robinet},
  journal={Human gene therapy methods},
  year={2015},
  volume={26 6},
  pages={
          197-210
        }
}
We evaluated the possibility of introducing a combination of six oncogenes into primary porcine hepatocytes (PPH) using a lentiviral vector (LV)-mediated gene transfer in order to develop a porcine hepatocellular carcinoma model based on autologous transplantation of ex vivo-transformed hepatocytes. The six oncogenes were introduced into three plasmids, hence enabling the production of LVs encoding a luciferase reporter gene and hTERT+p53(DD), cyclinD1+CDK4(R24C), and c-myc(T58A)+HRas(G21V… CONTINUE READING
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