Use of a Cell Recycle Reactor to Increase Production of a Proteolysis-Susceptible Peptide Secreted from Recombinant Saccharomyces cerevisiae

Abstract

Operation of a continuous microbial fermentor with cell recycle can significantly reduce degradation-associated loss of a secreted protein product. Under continuous fermentation conditions, proteolysis of a recombinant growth hormone releasing factor (GRF) analog secreted by S. cerevisiae was first order with respect to GRF concentration. The maximal GRF… (More)
DOI: 10.1038/nbt0790-639

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