Use of Unamplified RNA/cDNA–Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses

Abstract

Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of virus infections. Using rapid sequencing of unamplified RNA/cDNA hybrids, we identified Venezuelan equine encephalitis virus and Ebola virus in 3 hours from sample receipt to data acquisition, demonstrating a fieldable technique for RNA virus characterization.

DOI: 10.3201/eid2208.160270

Cite this paper

@inproceedings{Kilianski2016UseOU, title={Use of Unamplified RNA/cDNA–Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses}, author={Andy Kilianski and Pierce Roth and Alvin T. Liem and Jessica M. Hill and Kristen L. Willis and Rebecca D. Rossmaier and Andrew V. Marinich and Michele N. Maughan and Mark A. Karavis and Jens H Kuhn and Anna N Honko and C. Nicole Rosenzweig}, booktitle={Emerging infectious diseases}, year={2016} }