Use of Toxoplasma gondii expressing beta-galactosidase for colorimetric assessment of drug activity in vitro.

@article{McFadden1997UseOT,
  title={Use of Toxoplasma gondii expressing beta-galactosidase for colorimetric assessment of drug activity in vitro.},
  author={Diane C. McFadden and Franziska Seeber and John C Boothroyd},
  journal={Antimicrobial agents and chemotherapy},
  year={1997},
  volume={41 9},
  pages={1849-53}
}
A microtiter assay for drug evaluation has been developed with a strain of Toxoplasma gondii that expresses bacterial beta-galactosidase. By using chlorophenol red-beta-D-galactopyranoside (CPRG) as the substrate for beta-galactosidase, the efficacy of a drug against the parasite can be determined with a colorimetric readout. Drugs known to have activity against T. gondii (specifically, pyrimethamine, sulfadiazine, atovaquone, and clindamycin) were tested, and efficacies were determined by CPRG… CONTINUE READING

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