Use of Single Molecule Fluorescence Polarization Microscopy to Study Protein Conformation and Dynamics of Kinesin-Microtubule Complexes.

Abstract

Single molecule fluorescence polarization microscopy (smFPM) is a technique that enables to monitor changes in the orientation of a single labeled protein domain. Here we describe a smFPM microscope set-up and protocols to investigate conformational changes associated with the movement of motor proteins along cytoskeletal tracks. 
DOI: 10.1007/978-1-4939-7271-5_11

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Cite this paper

@article{Benoit2018UseOS, title={Use of Single Molecule Fluorescence Polarization Microscopy to Study Protein Conformation and Dynamics of Kinesin-Microtubule Complexes.}, author={Matthieu P M H Benoit and Hernando J. Sosa}, journal={Methods in molecular biology}, year={2018}, volume={1665}, pages={199-216} }