Use of RNA arbitrarily primed-PCR fingerprinting to identify Vibrio cholerae genes differentially expressed in the host following infection.

@article{Chakrabortty2000UseOR,
  title={Use of RNA arbitrarily primed-PCR fingerprinting to identify Vibrio cholerae genes differentially expressed in the host following infection.},
  author={Amit Chakrabortty and Siddhartha Das and Subrata Majumdar and Kanchan Mukhopadhyay and Siddhartha Roychoudhury and Keya Chaudhuri},
  journal={Infection and immunity},
  year={2000},
  volume={68 7},
  pages={3878-87}
}
Evidence suggests that a repertoire of Vibrio cholerae genes are differentially expressed in vivo, and regulation of virulence factors in vivo may follow a different pathway. Our work was aimed at characterization of in vivo-grown bacteria and identification of genes that are differentially expressed following infection by RNA arbitrarily primed (RAP)-PCR fingerprinting. The ligated rabbit ileal loop model was used. The motility of in vivo-grown bacteria increased by 350% over that of in vitro… CONTINUE READING

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