Use of RFLP and SSCP analysis to differentiate the pine rusts Cronartium flaccidum and Peridermium pini

@article{Moricca1998UseOR,
  title={Use of RFLP and SSCP analysis to differentiate the pine rusts Cronartium flaccidum and Peridermium pini},
  author={Salvatore Moricca and Alessandro Ragazzi},
  journal={Fungal Biology},
  year={1998},
  volume={102},
  pages={666-670}
}
Two rapid, independent molecular assays have been developed for discriminating between the closely related rusts of hard pines, Cronartium flaccidum and Peridermium pini . A portion of the ITS region and a portion of the IGS region from the ribosomal RNA operon of the two organisms were amplified with the PCR. Amplifications were made using DNA extracted from aeciospores collected from unopened aecia, taking a small number of spores as a source of template DNA. Amplified fragments were… Expand
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