Use of Peptide library screening to detect a previously unknown linear diagnostic epitope: proof of principle by use of lyme disease sera.

@article{Hamby2005UseOP,
  title={Use of Peptide library screening to detect a previously unknown linear diagnostic epitope: proof of principle by use of lyme disease sera.},
  author={Carl V. Hamby and Marta Llibre and Sandeepa Utpat and Gary P. Wormser},
  journal={Clinical and diagnostic laboratory immunology},
  year={2005},
  volume={12 7},
  pages={801-7}
}
Diagnostic peptides previously isolated from phage-displayed libraries by affinity selection with serum antibodies from patients with Lyme disease were found to give reproducible serum reactivity patterns when tested in two different enzyme-linked immunosorbent assay formats. In addition, the hypothetical possibility that peptides selected by this type of "epitope discovery" technique might identify the original antigens eliciting antibody responses was tested by searching for sequence… CONTINUE READING