Use of PCR for diagnosis of post-kala-azar dermal leishmaniasis.

  title={Use of PCR for diagnosis of post-kala-azar dermal leishmaniasis.},
  author={Omran Fadl Osman and Linda Oskam and Nel C M Kroon and Gerard J. Schoone and El Tahir A G Khalil and Ahmed Mohamed El-Hassan and Eduard Evert Zijlstra and Piet A. Kager},
  journal={Journal of clinical microbiology},
  volume={36 6},
Microscopy and PCR were compared for use in the diagnosis of post-kala-azar dermal leishmaniasis (PKDL) in 63 patients. Aspirates of lymph nodes (samples from 52 patients), skin (23 samples), and bone marrow (18 samples) were used. For 11 patients lymph node aspiration could be repeated 6 months after they recovered from PKDL. During active PKDL, PCR was positive for 42 of 52 (80.8%) lymph node aspirates and 19 of 23 (82.7%) skin aspirates, whereas microscopy was positive for only 9 of 52 (17.3… CONTINUE READING


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