Use of Ly6G‐specific monoclonal antibody to deplete neutrophils in mice

  title={Use of Ly6G‐specific monoclonal antibody to deplete neutrophils in mice},
  author={Jean M. Daley and Alan A. Thomay and Michael D. Connolly and Jonathan S. Reichner and Jorge E. Albina},
  journal={Journal of Leukocyte Biology},
The anti‐granulocyte receptor‐1 (Gr‐1) mAb, RB6‐8C5, has been used extensively to deplete neutrophils in mice and to investigate the role of these cells in host defense. RB6‐8C5 binds to Ly6G, which is present on neutrophils, and to Ly6C, which is expressed on neutrophils, dendritic cells, and subpopulations of lymphocytes and monocytes. It is thus likely that in vivo administration of RB6‐8C5 may deplete not only neutrophils but also other Gr‐l+ (Ly6C+) cells. This study describes the use of… 

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Durable and controlled depletion of neutrophils in mice

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Survival of residual neutrophils and accelerated myelopoiesis limit the efficacy of antibody‐mediated depletion of Ly‐6G+ cells in tumor‐bearing mice

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The present findings suggest that the Ly‐6C antigen family, shown by others to be involved in T cell activation, may have more general importance in immune responses and cellular differentiation than previously appreciated.

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Substantial CD8+ T cell responses are generated after infection of mice with recombinant Listeria monocytogenes strains expressing a model epitope (lymphocytic choriomeningitis virus NP118–126) in

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Absence of cross‐reactivity between murine Ly‐6C and Ly‐6G

Whether anti‐Ly‐6G antibodies truly cross‐react with the Ly‐6C antigen on multiple hematopoietic cell types is determined.

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