Use of Hydrogenophaga pseudoflava penetration to quantitatively assess the impact of filtration parameters for 0.2-micrometer-pore-size filters.

Abstract

Filters rated as having a 0.2-microm pore size (0.2-microm-rated filters) are used in laboratory and manufacturing settings for diverse applications of bacterial and particle removal from process fluids, analytical test articles, and gasses. Using Hydrogenophaga pseudoflava, a diminutive bacterium with an unusual geometry (i.e., it is very thin), we evaluated passage through 0.2-microm-rated filters and the impact of filtration process parameters and bacterial challenge density. We show that consistent H. pseudoflava passage occurs through 0.2-microm-rated filters. This is in contrast to an absence of significant passage of nutritionally challenged bacteria that are of similar size (i.e., hydrodynamic diameter) but dissimilar geometry.

DOI: 10.1128/AEM.01825-09

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Cite this paper

@article{Lee2010UseOH, title={Use of Hydrogenophaga pseudoflava penetration to quantitatively assess the impact of filtration parameters for 0.2-micrometer-pore-size filters.}, author={Aleck Lee and Jean McVey and Patrick J. Faustino and Sagar Lute and Nicholas Sweeney and Vishwajeet Pawar and Md . Ahaduzzaman Khan and Kurt A. Brorson and David Hussong}, journal={Applied and environmental microbiology}, year={2010}, volume={76 3}, pages={695-700} }