Use of HDEL-tagged Trichoderma reesei mannosyl oligosaccharide 1,2-alpha-D-mannosidase for N-glycan engineering in Pichia pastoris.

Abstract

Therapeutic glycoprotein production in the widely used expression host Pichia pastoris is hampered by the differences in the protein-linked carbohydrate biosynthesis between this yeast and the target organisms such as man. A significant step towards the generation of human-compatible N-glycans in this organism is the conversion of the yeast-type high… (More)

Topics

Cite this paper

@article{Callewaert2001UseOH, title={Use of HDEL-tagged Trichoderma reesei mannosyl oligosaccharide 1,2-alpha-D-mannosidase for N-glycan engineering in Pichia pastoris.}, author={Nico Callewaert and Wouter Laroy and H Cadirgi and Stijn Geysens and Xavier Saelens and Willy Min Jou and R. Contreras}, journal={FEBS letters}, year={2001}, volume={503 2-3}, pages={173-8} }