Use of HCA in subproteome-immunization and screening of hybridoma supernatants to define distinct antibody binding patterns.

@article{Szafran2016UseOH,
  title={Use of HCA in subproteome-immunization and screening of hybridoma supernatants to define distinct antibody binding patterns.},
  author={Adam T. Szafran and Maureen G. Mancini and Jeffrey A. Nickerson and Dean P. Edwards and Michael A. Mancini},
  journal={Methods},
  year={2016},
  volume={96},
  pages={75-84}
}
Understanding the properties and functions of complex biological systems depends upon knowing the proteins present and the interactions between them. Recent advances in mass spectrometry have given us greater insights into the participating proteomes, however, monoclonal antibodies remain key to understanding the structures, functions, locations and… CONTINUE READING