Use of GRA6-derived synthetic polymorphic peptides in an immunoenzymatic assay to serotype Toxoplasma gondii in human serum samples collected from three continents.

@article{Sousa2008UseOG,
  title={Use of GRA6-derived synthetic polymorphic peptides in an immunoenzymatic assay to serotype Toxoplasma gondii in human serum samples collected from three continents.},
  author={Susana Gama de Sousa and Daniel Ajzenberg and Manuel Vilanova and Jos{\'e} Sebasti{\'a}n D{\'a}vila Costa and M L Dard{\'e}},
  journal={Clinical and vaccine immunology : CVI},
  year={2008},
  volume={15 9},
  pages={1380-6}
}
Serotyping is a simple typing method that consists of an immunoenzymatic assay (enzyme-linked immunosorbent assay [ELISA]) using synthetic polymorphic peptides derived from Toxoplasma gondii antigens. We developed a new ELISA based on GRA6 C-terminal polymorphic peptides. Serum samples from 41 human infections due to 23 archetypal (type I, II, or III) and 18 nonarchetypal strains were selected in order to validate this approach. For 20 out of the 23 archetypal infections, there was a clear… CONTINUE READING
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