Use of Frog Eggs and Oocytes for the Study of Messenger RNA and its Translation in Living Cells

@article{Gurdon1971UseOF,
  title={Use of Frog Eggs and Oocytes for the Study of Messenger RNA and its Translation in Living Cells},
  author={J. Gurdon and C. Lane and H. Woodland and G. Marbaix},
  journal={Nature},
  year={1971},
  volume={233},
  pages={177-182}
}
Injected frog eggs and oocytes provide a very sensitive assay system for the identification of messenger RNA and permit the study of translational control in living cells. The translation of each haemoglobin messenger RNA molecule once every 5–10 minutes for at least 24 hours makes it possible to recognize less than 1 ng of this messenger RNA. 
Oocyte expression with injection of purified T7 RNA polymerase.
Translation of messenger RNA specific for tyrosine aminotransferase in oocytes of Xenopus laevis.
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