Use of Frog Eggs and Oocytes for the Study of Messenger RNA and its Translation in Living Cells

  title={Use of Frog Eggs and Oocytes for the Study of Messenger RNA and its Translation in Living Cells},
  author={J. B. Gurdon and Charles D. Lane and Hugh R. Woodland and G{\'e}rard Marbaix},
Injected frog eggs and oocytes provide a very sensitive assay system for the identification of messenger RNA and permit the study of translational control in living cells. The translation of each haemoglobin messenger RNA molecule once every 5–10 minutes for at least 24 hours makes it possible to recognize less than 1 ng of this messenger RNA. 

Injected nuclei in frog oocytes provide a living cell system for the study of transcriptional control

Human HeLa nuclei injected into Xenopus oocytes synthesise RNA continuously for up to 1 month, and some of this RNA is translated into HeLa proteins, which can be used for the study of transcriptional controls.

Translation of plant messengers in egg cells of Xenopus laevis

The results of experiments aimed at the use of this method for studying plant messenger RNA in living egg cells and oocytes of the tadpole of Xenopus laevis1–6 are described.

Collagen Synthesis in Xenopus Oocytes after Injection of Nuclear RNA of Frog Embryos

A messenger RNA fraction from polysomes of frog larvae or RNA preparations from isolated nuclei of developing frog embryos were injected into growing Xenopus laevis oocytes that were incubated with labeled proline, indicating that the injected material had promoted collagen synthesis.

Oocyte expression with injection of purified T7 RNA polymerase.

This work reports on a third expression technique that is based on the combined injection of cDNA and purified T7 RNA polymerase directly into the cytoplasm of oocytes.

Expression of Messenger RNAs Injected into Xenopus Laevis Oocytes

The genetic information carried by eukaryotic messenger RNAs can be translated to polypeptides either in cell-free protein synthesizing systems or after microinjection into living cells.

A microsystem for the extraction and in-vitro translation of mouse embryo mRNA.

A micromethod is described for the extraction of mRNA from mouse ova and its translation in a message-dependent rabbit reticulocyte lysate system and it has been possible to visualize the products from the equivalent of 12.5 eggs by SDS polyacrylamide gel fluorography.

Mouse oocytes transcribe injected Xenopus 5S RNA gene.

Transcripts produced after injection of the Xenopus 5S RNA gene into oocyte germinal vesicles of mice migrate electrophoretically with the 5S RNA marker, an indication that the gene is transcribed



Synthesis of a mouse immunoglobulin light chain in a rabbit reticulocyte cell-free system.

An RNA fraction isolated from a mouse myeloma functions as template for the synthesis of a mouse Ig light chain in a cell free system from rabbit reticulocytes.

Diffuse Interstellar Features in the Spectrum of the Most Heavily Reddened Star

THE Cygnus OB2 stellar association contains the most heavily reddened early type star known1,2. If all interstellar grains were to be removed from the line of sight to this eleventh magnitude star,

Isolation of an RNA with the Properties of Haemoglobin Messenger

An RNA species with properties suggesting that it is a messenger for haemoglobin synthesis has been purified from rabbit reticulocytes. It resembles messenger RNA in its molecular size, its

Changes in somatic cell nuclei inserted into growing and maturing amphibian oocytes.

  • J. Gurdon
  • Biology
    Journal of embryology and experimental morphology
  • 1968
The experiments to be reported here were designed to find out whether the same generalizations apply to nuclei inserted into cells other than eggs, and whether they are true of changes in nuclear activity other than the repression of RNA synthesis and the induction of DNA synthesis.