Use of Droplet Digital PCR for Estimation of Fish Abundance and Biomass in Environmental DNA Surveys

  title={Use of Droplet Digital PCR for Estimation of Fish Abundance and Biomass in Environmental DNA Surveys},
  author={Hideyuki Doi and Kimiko Uchii and Teruhiko Takahara and Saeko Matsuhashi and Hiroki Yamanaka and Toshifumi Minamoto},
  booktitle={PloS one},
An environmental DNA (eDNA) analysis method has been recently developed to estimate the distribution of aquatic animals by quantifying the number of target DNA copies with quantitative real-time PCR (qPCR). A new quantitative PCR technology, droplet digital PCR (ddPCR), partitions PCR reactions into thousands of droplets and detects the amplification in each droplet, thereby allowing direct quantification of target DNA. We evaluated the quantification accuracy of qPCR and ddPCR to estimate… CONTINUE READING
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