Use of CRISPR-modified human stem cell organoids to study the origin of mutational signatures in cancer

@article{Drost2017UseOC,
  title={Use of CRISPR-modified human stem cell organoids to study the origin of mutational signatures in cancer},
  author={Jarno Drost and Ruben van Boxtel and Francis Blokzijl and Tomohiro Mizutani and Nobuo Sasaki and Valentina Sasselli and Joep de Ligt and Sam Behjati and Judith E Grolleman and Tom van Wezel and Serena Nik-Zainal and Roland P Kuiper and Edwin Cuppen and Hans Clevers},
  journal={Science},
  year={2017},
  volume={358},
  pages={234-238}
}
Mutational processes underlie cancer initiation and progression. Signatures of these processes in cancer genomes may explain cancer etiology and could hold diagnostic and prognostic value. We developed a strategy that can be used to explore the origin of cancer-associated mutational signatures. We used CRISPR-Cas9 technology to delete key DNA repair genes in human colon organoids, followed by delayed subcloning and whole-genome sequencing. We found that mutation accumulation in organoids… CONTINUE READING

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Organoid technology and applications in cancer research

Journal of hematology & oncology • 2018
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