Use of 5-cyano-2,3-ditolyl-tetrazolium chloride staining as an indicator of biocidal activity in a rapid assay for anti-Acanthamoeba agents.

@article{Kobayashi2012UseO5,
  title={Use of 5-cyano-2,3-ditolyl-tetrazolium chloride staining as an indicator of biocidal activity in a rapid assay for anti-Acanthamoeba agents.},
  author={Takeshi Kobayashi and Tsuyoshi Mito and Narumi Watanabe and Takashi Suzuki and Atsushi Shiraishi and Yuichi Ohashi},
  journal={Journal of clinical microbiology},
  year={2012},
  volume={50 5},
  pages={1606-12}
}
The usefulness of 5-cyano-2,3-ditolyl-tetrazolium chloride (CTC) staining to determine the respiratory activity of Acanthamoeba was evaluated in this study. Acanthamoeba trophozoites and cysts have a red fluorescence after staining with CTC. To determine the effectiveness of CTC staining as a CTC biocidal assay for Acanthamoeba, the trophozoites and cysts of Acanthamoeba castellanii (ATCC 5037) were treated with serial concentrations of disinfectant solutions, namely, polyhexamethylene… CONTINUE READING