Use of 2n gametes for the production of sexual polyploids from sterile Oriental × Asiatic hybrids of lilies (Lilium)

  title={Use of 2n gametes for the production of sexual polyploids from sterile Oriental × Asiatic hybrids of lilies (Lilium)},
  author={Rodrigo Barba-Gonz{\'a}lez and Andrew Lokker and Ki-Byung Lim and Munikote S. Ramanna and Jaap M. van Tuyl},
  journal={Theoretical and Applied Genetics},
Sixteen Oriental and 12 Asiatic cultivars were crossed in 158 different combinations. A total of 708 F1 hybrids were obtained from 86 of the different combinations of 15 Oriental and 11 Asiatic cultivars. Because the Lilium cultivars (2n=2x=24) used for the production of these hybrids belong to two different taxonomic sections—Archelirion (O) and Sinomartagon (A), respectively—the F1 hybrids (OA) could be obtained only through embryo, embryo sac rescue, ovary slice or ovule culture. Most of the… 

Occurrence of 2n gametes in the F1 hybrids of Oriental × Asiatic lilies (Lilium): Relevance to intergenomic recombination and backcrossing

Based on in vitro pollen germination it was proved for two genotypes that 2n pollen was viable only during the first day of anthesis, and it was possible to use 1n pollen successfully for backcrossing and for intergenomic recombination in BC1 progenies.

The use of 2n gametes for introgression breeding in Oriental x Asiatic lilies

A molecular cytogenetic investigation was conducted on the possibilities of introgression of characters between Oriental and Asiatic lilies through the use of 2n gametes, confirming the presence of both O and A genome chromosomes with some of them possessing recombinant segments.

Genome composition of triploid lily cultivars derived from sexual polyploidization of Longiflorum × Asiatic hybrids (Lilium)

About 19 cultivars, which had originated from backcrosses between F1 LA (Longiflorum × Asiatic) hybrids as female parents and Asiatic cultivars as male parents, were analyzed with genomic in situ hybridization and showed that intergenomic recombination might play an important role during the selection of the cultivars directly from BC1 progenies.

Production of Polyploids and Unreduced Gametes in Lilium auratum × L. henryi Hybrid

The results of this study indicate that production and analysis of F1 AuH hybrids and their progeny through sexual polyploidization can be useful for efficient creation of important horticultural traits.

Relevance of unilateral and bilateral sexual polyploidization in relation to intergenomic recombination and introgression in Lilium species hybrids

The nature of interspecific lily hybrids obtained from uni- and bilateral sexual polyploidization leading to allotriploid and allotetraploid formation is discussed in the context of introgression and intergenomic recombination.

Intergenomic recombination in F1 lily hybrids (Lilium) and its significance for genetic variation in the BC1 progenies as revealed by GISH and FISH.

Intergenomic recombination was assessed in a BC1 population of Oriental (O)xAsiatic (A) lilies (Lilium) backcrossed to Asiatic parents, and an important feature of A x OA backcross progeny is the occurrence of substitutions for the segment distal in the crossover wherever the recombinant chromosome O/A was present.

Progenies of allotriploids of Oriental × Asiatic lilies (Lilium) examined by GISH analysis

With the aim of utilizing allotriploid Asiatic lily hybrids (Lilium) in introgression breeding, different types of crosses were made with two types of triploids used as parents, derived from: (a) mitotic polyploidization and (b) sexual polyploidsization.

A molecular cytogenetic study of intergenomic recombination and introgression of chromosomal segments in lilies (Lilium)

A molecular cytogenetic investigation was conducted to evaluate the amount of recombination and introgression of characters between Longiflorum - Asiatic (LA) and Oriental - As iatic (OA) hybrids through the use of n and 2n gametes.

A Molecular Cytogenetic Analysis of Introgression in Backcross Progenies of Intersectional Lilium Hybrids

Based on the cytological analysis of progenies derived from the use of both haploid and 2n gametes, cytological maps of three different genomes have been constructed and the relevance of these analyses for introgression in Lilium is demonstrated.



Occurrence of numerically unreduced (2n) gametes in Alstroemeria interspecific hybrids and their significance for sexual polyploidisation

It was concluded that in allopolyploids of Alstroemeria, bilateral sexual polyploidisation could accomplish genetic recombination by both homoeologous crossing-over as well as through the assortment of chromosomes.

Homoeologous recombination in 2n-gametes producing interspecific hybrids of Lilium (Liliaceae) studied by genomic in situ hybridization (GISH)

A clear differentiation between the chromosomes of L. longiflorum, Asiatic, and Oriental hybrids was observed in four ALA- and one OLA-hybrid using GISH, and evidence for the occurrence of the FDR-mechanism was found.

Fertility and meiotic mechanisms of hybrids between chromosome autoduplication tetraploid wheats and Aegilops species

The present study revealed that the autoduplication of chromosomes in these F1's was caused by the tetraploid wheat parents T. persicum PS5 and T. durum DR147.

Introgression of Lilium Rubellum Baker Chromosomes into L. Longiflorum Thunb.: A Genome Painting Study of the F1 Hybrid, BC1 and BC2 Progenies

It seems that the preferential pairing in the F1 and BC1 hybrids hinder the introgression of the chromosome segments or species-specific genes into the recipient for breeding purposes.

Evaluation of BC2 progenies derived from 3x-2x and 3x-4x crosses of Lilium hybrids: a GISH analysis

An allotriploid BC1 plant was obtained by backcrossing a diploid F1 interspecific hybrid derived from a Lilium longiflorum and an Asiatic hybrid, and the homoeologous recombinant chromosomes were transmitted intact from BC1 to BC2 progenies in variable numbers.

The extent and position of homoeologous recombination in a distant hybrid of Alstroemeria: a molecular cytogenetic assessment of first generation backcross progenies

Besides homeologous recombination, evidence was found for the presence of structural rearrangements (inversion and translocation) between the chromosomes of the parental species and the ancient translocation was confirmed through FISH analysis of mitotic and meiotic chromosomes.

Relevance of sexual polyploidization for crop improvement – A review

Methods have become available for the analysis of 2ngametes and sexual polyploid progenies moreaccurately and to develop systematicbreeding approaches, which can be potentially useful for a more efficient creation of desirable breeding material andcultivars.

The use of genomic in situ hybridization (GISH) to show transmission of recombinant chromosomes by a partially fertile bigeneric hybrid, Gasteria lutzii ×Aloe aristata (Aloaceae), to its progeny

The discrepancy suggests that terminalization may occur prior to or during metaphase I, reducing the apparent chiasma frequency, or possibly reveals an under-estimation caused by difficulties in resolving closely grouped chiasmata by eye.

Indeterminate meiotic restitution (IMR): a novel type of meiotic nuclear restitution mechanism detected in interspecific lily hybrids by GISH

This novel type of restitution mechanism is indicated as Indeterminate Meiotic Restitution (IMR) and the chromosome constitutions of eight triploid BC1 progenies derived from backcrossing the 2n-gamete producing the LAhybrids to the Asiatic hybrid parents were analysed through in situ hybridisation.

First-division restitution in hybrids of Langdon durum disomic substitution lines with rye and Aegilops squarrosa

The results suggest that the reduced or absent FDR in such hybrids might be related to the substitution of chromosomes with an FDR gene and poor compensation ability of the D-genome chromosomes for their homoeologous A- or B- genome chromosomes.