Use of 2 pneumococcal common protein real-time polymerase chain reaction assays in healthy children colonized with Streptococcus pneumoniae.

@article{Rouphael2011UseO2,
  title={Use of 2 pneumococcal common protein real-time polymerase chain reaction assays in healthy children colonized with Streptococcus pneumoniae.},
  author={Nadine Georges Rouphael and Sanet Steyn and Mathieu Bangert and Jackie S Sampson and Peter Adrian and Shabir A Madhi and Keith P. Klugman and Edwin W. Ades},
  journal={Diagnostic microbiology and infectious disease},
  year={2011},
  volume={70 4},
  pages={452-4}
}
Polymerase chain reaction (PCR) offers promise in pneumococcal diagnostics, but whether nasopharyngeal carriage causes false-positive results in people colonized with Streptococcus pneumoniae is unknown. We found in serum no positive samples for pneumococcal DNA in 100 carriers and noncarriers using 2 different real-time PCR assays targeting lytA and psaA genes. 

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