Urokinase directly activates matrix metalloproteinases-9: a potential role in glioblastoma invasion.

@article{Zhao2008UrokinaseDA,
  title={Urokinase directly activates matrix metalloproteinases-9: a potential role in glioblastoma invasion.},
  author={Yun-ge Zhao and Charles E. Lyons and Aizhen Xiao and Dennis J. Templeton and Qingxiang Amy Sang and Keith Brew and Isa Marte Hussaini},
  journal={Biochemical and biophysical research communications},
  year={2008},
  volume={369 4},
  pages={1215-20}
}
Previous reports showed that urokinase plasminogen activator (uPA) converts plasminogen to plasmin which then activates matrix metalloproteinases (MMPs). Here, we report that uPA directly cleaved pro-MMP-9 in a time-dependent manner at both C- and N-terminus and generated two gelatinolytic bands. uPA-activated-MMP-9 efficiently degraded fibronectin and blocked by uPA inhibitor B428 and recombinant tissue inhibitor of metalloproteinase-1 (TIMP-1). B428 inhibited basal and PMA-induced active MMP… CONTINUE READING