Urinary metabolic profiling of colorectal carcinoma based on online affinity solid phase extraction-high performance liquid chromatography and ultra performance liquid chromatography-mass spectrometry.

@article{Wang2010UrinaryMP,
  title={Urinary metabolic profiling of colorectal carcinoma based on online affinity solid phase extraction-high performance liquid chromatography and ultra performance liquid chromatography-mass spectrometry.},
  author={Wenzhao Wang and Bo Feng and Xiang Li and Peiyuan Yin and Peng Gao and Xinjie Zhao and Xin Lu and Minhua Zheng and Guowang Xu},
  journal={Molecular bioSystems},
  year={2010},
  volume={6 10},
  pages={
          1947-55
        }
}
Colorectal carcinoma (CRC) is the third most commonly encountered cancer and fourth cause of cancer-associated death worldwide. Abundant studies have demonstrated that one of the best effective therapies for enhancing the 5-year survival rate of patients is to diagnose the disease at an early stage. Urine metabonomics is widely being utilized as an efficient platform to investigate the metabolic changes and discover the potential biomarkers of malignant diseases. In this study both ultra… 
View on PubMed
rsc.org
40 Citations
Highly Influential Citations
1
Background Citations
8
Methods Citations
1

40 Citations

Citation Type

Citation Type

Urinary hydrophilic and hydrophobic metabolic profiling based on liquid chromatography‐mass spectrometry methods: Differential metabolite discovery specific to ovarian cancer
TLDR
The differences of urinary hydrophilic and hydrophobic metabolic profiling between healthy women, benign ovarian tumor, and ovarian cancer patients were studied by metabolomics strategy and five metabolites were found to be specific to ovarian cancer.
Urinary metabolomic study of non-small cell lung carcinoma based on ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.
TLDR
This study suggests that the developed carnitine and acylcarnitines profiling method has the potential to be used for screening non-small cell lung carcinoma.
Development and Validation of a High-Throughput Mass Spectrometry Based Urine Metabolomic Test for the Detection of Colonic Adenomatous Polyps
TLDR
A clinically scalable MS-based urine metabolomic test that identifies patients with colonic adenomatous polyps at a higher level of sensitivity (86%) over current fecal-based tests (<18%).
A METABONOMICS STUDY OF COLORECTAL CANCER BY RRLC-QTOF/MS
TLDR
It is suggested that CRC urinary metabolites might reflect specific tumor metabolisms and could be used as potential urinary metabolic biomarkers to reflect pathological changes of CRC.
Ultra-performance liquid chromatography coupled to mass spectrometry as a sensitive and powerful technology for metabolomic studies.
TLDR
Hyphenated UPLC/MS technique is becoming a useful tool in the study of body fluids, represents a promising hyphenated microseparation platform in metabolomics and has a strong potential to contribute to disease diagnosis.
Urine molecular profiling distinguishes health and disease: new methods in diagnostics? Focus on UPLC–MS
  • S. König
  • Biology
    Expert review of molecular diagnostics
  • 2011
TLDR
The combination of high-end analytical technology with multivariate statistics allows differential analysis of replicate samples with applications for both high- and low-molecular-weight analytes, namely proteins and metabolites.
Current practice of liquid chromatography-mass spectrometry in metabolomics and metabonomics.
Development of nanoflow liquid chromatography-nanoelectrospray ionization mass spectrometry methodology for improved urine metabolomics
TLDR
Methods were developed to use nanoflow ultra high performance liquid chromatography-nanospray ionization-mass spectrometry (nUHPLC-nESI-TOFMS) analysis methods for metabolomic analyses of urine samples to investigate the metabolomic consequences of HIV infection and patient response to combined antiretroviral therapy (cART).
Changes in urinary metabolic profiles of colorectal cancer patients enrolled in a prospective cohort study (ColoCare)
TLDR
This is one of the first metabolomic studies to follow CRC patients in a prospective setting with repeated urine sampling over time and was able to confirm markers initially identified in case–control studies and metabolites which may represent prognostic biomarker candidates of CRC.
Metabolomics Biomarkers for Detection of Colorectal Neoplasms: A Systematic Review
TLDR
Panels of metabolites consisting of amino acids in blood and nucleosides in urinary samples might be useful biomarkers for early detection of advanced colorectal neoplasms, however, to make metabolomic biomarkers clinically applicable, future research in larger studies and external validation of the results is required.
...
1
2
3
4
...

References

SHOWING 1-10 OF 46 REFERENCES
Ultra-High Performance Liquid Chromatography–Mass Spectrometry for the Metabolomic Analysis of Urine in Colorectal Cancer
TLDR
Preliminary results suggest that the UPLC–MS-based method coupled with pattern recognition will likely lead to procedures with the potential to be clinically applicable for the diagnosis of CRC and, consequently, to an improvement in patient prognosis.
Metabonomics study of liver cancer based on ultra performance liquid chromatography coupled to mass spectrometry with HILIC and RPLC separations.
Metabolic profiling of human colorectal cancer using high-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy and gas chromatography mass spectrometry (GC/MS).
Current clinical strategy for staging and prognostication of colorectal cancer (CRC) relies mainly upon the TNM or Duke system. This clinicopathological stage is a crude prognostic guide because it…
Analysis of urinary nucleosides as potential tumor markers in human colorectal cancer by high performance liquid chromatography/electrospray ionization tandem mass spectrometry.
A pilot study of gas chromatograph/mass spectrometry-based serum metabolic profiling of colorectal cancer after operation
TLDR
The result demonstrated the GC-MS technique is an valuable tool for the characterization of the metabolic perturbation, and the metabolomic study will certainly benefit for monitoring the nutrition state of CRC patients, the prognosis and therapy evaluation of CRC Patients after operation.
Reduced levels of hydroxylated, polyunsaturated ultra long-chain fatty acids in the serum of colorectal cancer patients: implications for early screening and detection
TLDR
A novel comprehensive metabolomics technology was used to identify a systemic metabolic dysregulation comprising previously unknown hydroxylated polyunsaturated ultra-long chain fatty acid metabolites in CRC patients.
Urinary metabonomic study on colorectal cancer.
TLDR
This study assessed the important metabonomic variations in urine associated with CRC and provided baseline information complementary to serum/plasma and tissue metabonomics for the complete elucidation of the underlying metabolic mechanisms of CRC.
Metabolite profiling of fecal water extracts from human colorectal cancer
TLDR
The results show that metabolic profiling of fecal water extracts is a cheap, reproducible and effective method for detecting colorectal cancer markers and therefore complements other stool‐screening methods.
Quantitative metabolome profiling of colon and stomach cancer microenvironment by capillary electrophoresis time-of-flight mass spectrometry.
TLDR
Capillary electrophoresis coupled to mass spectrometry-based metabolomics, which is capable of quantifying the levels of energy metabolites in tissues, could be a powerful tool for the development of novel anticancer agents that target cancer-specific metabolism.
Analysis of urinary nucleosides as helper tumor markers in hepatocellular carcinoma diagnosis.
TLDR
Combined with the determination of serum AFP levels, the higher levels of urinary adenosine, cytidine, and inosine may be additional diagnosis markers for HCC in Taiwanese patients.
...
1
2
3
4
5
...