Urinary marker of oral pregnenolone administration

  title={Urinary marker of oral pregnenolone administration},
  author={Christophe M. Saudan and Aur{\'e}lien Desmarchelier and Pierre-Edouard Sottas and Patrice Mangin and Martial Saugy},

Investigations on changes in ¹³C/¹²C ratios of endogenous urinary steroids after pregnenolone administration.

Due to its properties to disguise the misuse of anabolic steroids by influencing the ¹³C/¹²C ratio of PD, PREG should be considered to be added to the World Anti-Doping Agency (WADA) list of prohibited substances as a masking agent.

Impact of progesterone administration on doping test of endogenous steroids

The influences of oral and intramuscular administration of PROG on the urinary steroid profile and carbon isotope ratios of steroids were investigated and it was demonstrated that the urine concentrations and the δ13C-values of PD were affected obviously.

Investigations on hydrogen isotope ratios of endogenous urinary steroids: reference-population-based thresholds and proof-of-concept.

The obtained results enabled the calculation of the first reference-population-based thresholds for HIR of urinary steroids that can readily be applied to routine doping control samples and proved to be a new tool supporting modern sports drug testing procedures.

Carbon isotope ratio analysis of endogenous glucocorticoid urinary metabolites after cortisone acetate and adrenosterone administration for doping control.

This study reaffirms that CIR methods for the doping control of endogenous steroids should not rely upon a single ERC, as the administration of an appropriate precursor to that ERC could cause complications during analysis.

Carbon isotope ratio mass spectrometry for detection of endogenous steroid use: a testing strategy.

Isotope ratio mass spectrometry (IRMS) testing is performed to determine if an atypical steroid profile is due to administration of an endogenous steroid. Androsterone (Andro) and etiocholanolone

A simplified and accurate method for the analysis of urinary metabolites of testosterone-related steroids using gas chromatography/combustion/isotope ratio mass spectrometry.

These carefully controlled analytical conditions are compatible with routine operations, affording accurate and precise results for the more diagnostically relevant metabolites such as testosterone itself and the 5α- and 5β-androstanediols.

Testosterone and doping control

An overview of the use of anabolic-androgenic steroids in sport and methods used in anti-doping laboratories for their detection in urine, with special emphasis on doping with testosterone, is given.



Urinary analysis of four testosterone metabolites and pregnanediol by gas chromatography-combustion-isotope ratio mass spectrometry after oral administrations of testosterone.

Measurements of 5 beta-androstanediol delta-values allow the detection of a testosterone ingestion over a longer post-administration period than other T metabolites delta(13)C-values or than the usual T/E ratio approach.

16-ene-steroids in the human testis

  • A. SmalsJ. Weusten
  • Biology, Medicine
    The Journal of Steroid Biochemistry and Molecular Biology
  • 1991

Serum and urinary markers of exogenous testosterone administration

Assessment of porcine and human 16-ene-synthase, a third activity of P450c17, in the formation of an androstenol precursor. Role of recombinant cytochrome b5 and P450 reductase.

It is demonstrated that human P450c17, as other enzymes of the classical steroidogenic pathway, is involved in the biosynthetic pathway leading to the formation of androstenol.

Criteria to indicate testosterone administration.

This study showed that both T/E and T/LH ratios could be used to detect testosterone administration, the latter also being an indicator of HCG use due to cross-reactivity with the LH antiserum.