Urinary 8-hydroxydeoxyguanosine and its analogs as DNA marker of oxidative stress: development of an ELISA and measurement in both bladder and prostate cancers.

@article{Chiou2003Urinary8A,
  title={Urinary 8-hydroxydeoxyguanosine and its analogs as DNA marker of oxidative stress: development of an ELISA and measurement in both bladder and prostate cancers.},
  author={Chiuan Chian Chiou and Pi Yueh Chang and Err Cheng Chan and Tsu Lan Wu and Kuo Chien Tsao and James T. Wu},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={2003},
  volume={334 1-2},
  pages={87-94}
}
BACKGROUND 8-hydroxydeoxyguanosine (8-OHdG) is the most frequently detected and studied DNA lesion. Upon DNA repair, 8-OHdG is excreted in the urine. Urinary 8-OHdG is now considered as a biomarker of generalized, cellular oxidative stress and is linked to degenerative diseases including cancer. METHODS We developed a competitive enzyme-linked immunosorbent assay (ELISA) for urinary 8-OHdG by coating BSA conjugated 8-hydroxyguanine (8-OHG) on a microplate. Urine specimens containing 8-OHdG… CONTINUE READING